Hi Sam,
Welcome to the forum! I hope you stay around for a while. It's hard to find people who have some tangible experience with the Opae ula, so anything you can contribute is highly appreciated.
As for breeding these shrimp, it seems that they are highly adaptable with regards to salinity. I have larvae and juveniles (and ovigerous females) right now happily living and breeding at 20-21 ppt salinity (this is what I just measured). My salinity usually fluctuates between 14 and 23 ppt depending on evaporation. Lately I had been a little lax on topping off their tank so the salinity has been at the higher end for almost two months. During this time I saw two batches of larvae and some juveniles.
I just took a picture of a juvenile (only a few days old and about 3-4mm) that happened to be sitting right on the front glass of the tank "begging" to be photographed as I was reading this topic.
Here it is:
Sorry for the bad picture, but that's all I could muster given the conditions. The "baby" shrimp is actually redder in real live. The camera did not quite capture it.
I have had reproduction occuring at 8-12 ppt before also, which underscores this species' adaptability. It's interesting that you note that most of their natural habitats have salinities under 10 ppt. That begs the question how high the salinity in the deeper parts of the pools is as low salinity water usually sits on top of the high salinity water. Also, did anyone try to somehow measure the salinity in the subterranean habitats that connect these pools together and also connect them with the ocean?
It also seems likely that the larvae could take full ocean salinity, which would make it easier for them to colonize new habitats on "new islands" (geologically young) that do not have Opae ula populations yet. It seems more likely than the adults walking over to these islands on the ocean floor. That would also explain why Antecaridina lauensis (another red anchialine pool shrimp for those who wonder) can be found at such disjoint locations all over the world, including Hawaii, Solomon Islands, Japan, Madagascar, the Red Sea and even in the Atlantic (Bermuda). I understand that these might just be remnants of ancient populations (as the leading theory suggests), but still they need some way to move to new islands once these prop out of the ocean.
As to temperature, the temperature in their tank right now is 78 degrees, but I have had reproduction occur at 80-82 degrees also. This again underscores their ability to adjust to various conditions.
Finally, have you ever tried breeding these shrimp in hard, high PH freshwater? If you can breed them at 2-4 ppt you might actually be able to do the same at 0 ppt salinity. I am going to try it out at some point once my Opae population is large enough but I was just wondering if it had been tried before.
For such an interesting shrimp there seems to be very little research on this species.
Again, welcome to the forum and I am looking forward to your contributions.
Mustafa